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nitrocellulose membrane  (Bio-Rad)


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    Structured Review

    Bio-Rad nitrocellulose membrane
    Nitrocellulose Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 32487 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nitrocellulose+membrane/Nitrocellulose+Membrane/10__22175_slash_mmb__17646-66-22-24
    Average 99 stars, based on 32487 article reviews
    nitrocellulose membrane - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Membrane:

    Article Title: A CHK1-mediated phosphorylation switch suppresses human Topoisomerase 1-associated genomic instability.
    Article Snippet: .. DNA (500 ng per sample) was spotted on a nitrocellulose membrane using a slot blot apparatus (Bio-Rad, USA) under vacuum. .. The membrane was air-dried, sandwiched in Whatman Paper, and baked at 80 °C for 2 h in a hot air oven.

    Article Title: Title Pending 17646
    Article Snippet: Total protein content was estimated using the Bradford protein assay (Bio-Rad, Hercules, CA, USA) and stored at −80°C. .. The proteins were separated using a 10% SDS-PAGE gel loaded with 40 μg of protein per sample, transferred to a 0.45 μm Nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA), and blocked for 1 h at room temperature with 2% bovine serum albumin (BSA, Sigma Aldrich®) in 1x Tris-Buffered Saline (TBS1x; 50mM Tris-HCL, pH 7.5; 150mM NaCl; Sigma Aldrich®). ..

    Article Title: The role of ATP synthase subunit e (ATP5I) in mediating the metabolic and antiproliferative effects of metformin in cancer cells
    Article Snippet: .. A quantity of 25–40 μg of protein extracts was loaded into SDS–PAGE (12–15% for resolving gels, 4% for stacking gels) and run in Tris-Glycine SDS buffer (192 mM glycine, 0.1% SDS, 25 mM Tris-Base) at 90 V. SDS–PAGE were then transferred on a nitrocellulose membrane (0.45 μm, Bio-Rad, Mississauga, ON, Canada) in Tris-Glycine buffer (96 mM glycine, 10 mM Tris-Base) for 1h30 at 120 V. Membranes were blocked in a modified Tris-buffered saline + 0.05% Tween-20 (TBST) solution with 5% skim milk for 1 hr at rt, washed with TBST 3 × 5 min at rt, and were then incubated with an appropriately diluted solution of primary antibody (in 0.1% bovine serum albumin [BSA], 0.02% sodium azide, PBS pH 7.4) overnight (o/n) at 4°C. ..

    Article Title: The apical polar ring is essential during the blood stage of Plasmodium falciparum.
    Article Snippet: Parasite pellets were PBS-washed and boiled in 1× Laemmli sample buffer (Bio-Rad) with BME at 95 °C for 5 min. Proteins (1 × 108 parasites per lane) were resolved on 4–20% Tris–glycine–SDS gel (Bio-Rad; 120 V). .. Proteins were transferred overnight (30 V, 4 °C) to a nitrocellulose membrane (Bio-Rad) using a wet transfer system. .. Membranes were blocked with LI-COR Odyssey blocking buffer for 1 h at RT, probed with primary and IRDye-conjugated secondary antibodies (Supplementary Table 3).

    Article Title: WEE1 Stabilizes MYC to Promote Therapeutic Resistance in Esophageal Adenocarcinoma
    Article Snippet: The supernatant was transferred to new tubes, and the protein concentration of the cell lysates was estimated using the BCA kit (Pierce Biotechnology, Waltham, MA). .. 10μg of the protein lysates were subjected to SDS-PAGE, and the protein bands were transferred to a Nitrocellulose membrane (Bio-Rad, Hercules, CA). ..

    Article Title: An optimized translating ribosome affinity purification protocol for low-abundance Drosophila tissues
    Article Snippet: .. Lysates were equally loaded and separated by SDS-PAGE in 4–20 % Mini-PROTEAN® TGXTM Precast Protein Gels (Bio-Rad; 4561096) and transferred onto a nitrocellulose membrane (Bio-Rad; 1704158) via Trans-Blot Turbo Transfer System (Bio-Rad). .. Membranes were blocked for 1 hour at room temperature with 5 % (wt/vol) dried skimmed milk powder (Marvel Instant Milk) in TBS containing 0.1 % Tween-20 (TBS-T) and incubated overnight at 4 °C with an α-GFP (1:1000; Abcam; ab1218) or an α-Flag antibody (1:1000; Sigma-Aldrich; F1804).

    Article Title: Investigating the functional components of YAP condensates
    Article Snippet: Yes-associated protein (YAP) condensates are critical for cell survival under hyperosmotic stress, yet how these condensates execute their specific functions remains incompletely understood.. Here, we employed proximity-based proteomics to identify YAP-interacting proteins in both the diffuse and condensate-forming states.. Upon YAP condensate formation, the composition of YAPinteracting proteins changed markedly.

    Article Title: Use of tandem‐affinity chromatography to incorporate nucleotide loading of small GTPases during their purification
    Article Snippet: .. The next day, the nitrocellulose membrane was treated with Ponceau S stain and bands were recorded using a Bio‐Rad ChemiDoc. .. Ponceau S stain was removed from the membrane via a 0.1% NaOH solution, and then blocked for 5 min using 20 mL of Every‐Blot Blocking Buffer from Bio‐Rad.

    Dot Blot:

    Article Title: A CHK1-mediated phosphorylation switch suppresses human Topoisomerase 1-associated genomic instability.
    Article Snippet: .. DNA (500 ng per sample) was spotted on a nitrocellulose membrane using a slot blot apparatus (Bio-Rad, USA) under vacuum. .. The membrane was air-dried, sandwiched in Whatman Paper, and baked at 80 °C for 2 h in a hot air oven.

    SDS Page:

    Article Title: Title Pending 17646
    Article Snippet: Total protein content was estimated using the Bradford protein assay (Bio-Rad, Hercules, CA, USA) and stored at −80°C. .. The proteins were separated using a 10% SDS-PAGE gel loaded with 40 μg of protein per sample, transferred to a 0.45 μm Nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA), and blocked for 1 h at room temperature with 2% bovine serum albumin (BSA, Sigma Aldrich®) in 1x Tris-Buffered Saline (TBS1x; 50mM Tris-HCL, pH 7.5; 150mM NaCl; Sigma Aldrich®). ..

    Article Title: An optimized translating ribosome affinity purification protocol for low-abundance Drosophila tissues
    Article Snippet: .. Lysates were equally loaded and separated by SDS-PAGE in 4–20 % Mini-PROTEAN® TGXTM Precast Protein Gels (Bio-Rad; 4561096) and transferred onto a nitrocellulose membrane (Bio-Rad; 1704158) via Trans-Blot Turbo Transfer System (Bio-Rad). .. Membranes were blocked for 1 hour at room temperature with 5 % (wt/vol) dried skimmed milk powder (Marvel Instant Milk) in TBS containing 0.1 % Tween-20 (TBS-T) and incubated overnight at 4 °C with an α-GFP (1:1000; Abcam; ab1218) or an α-Flag antibody (1:1000; Sigma-Aldrich; F1804).

    Semi-Dry Trans-Blot:

    Article Title: Title Pending 17646
    Article Snippet: Total protein content was estimated using the Bradford protein assay (Bio-Rad, Hercules, CA, USA) and stored at −80°C. .. The proteins were separated using a 10% SDS-PAGE gel loaded with 40 μg of protein per sample, transferred to a 0.45 μm Nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA), and blocked for 1 h at room temperature with 2% bovine serum albumin (BSA, Sigma Aldrich®) in 1x Tris-Buffered Saline (TBS1x; 50mM Tris-HCL, pH 7.5; 150mM NaCl; Sigma Aldrich®). ..

    Saline:

    Article Title: Title Pending 17646
    Article Snippet: Total protein content was estimated using the Bradford protein assay (Bio-Rad, Hercules, CA, USA) and stored at −80°C. .. The proteins were separated using a 10% SDS-PAGE gel loaded with 40 μg of protein per sample, transferred to a 0.45 μm Nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA), and blocked for 1 h at room temperature with 2% bovine serum albumin (BSA, Sigma Aldrich®) in 1x Tris-Buffered Saline (TBS1x; 50mM Tris-HCL, pH 7.5; 150mM NaCl; Sigma Aldrich®). ..

    Article Title: The role of ATP synthase subunit e (ATP5I) in mediating the metabolic and antiproliferative effects of metformin in cancer cells
    Article Snippet: .. A quantity of 25–40 μg of protein extracts was loaded into SDS–PAGE (12–15% for resolving gels, 4% for stacking gels) and run in Tris-Glycine SDS buffer (192 mM glycine, 0.1% SDS, 25 mM Tris-Base) at 90 V. SDS–PAGE were then transferred on a nitrocellulose membrane (0.45 μm, Bio-Rad, Mississauga, ON, Canada) in Tris-Glycine buffer (96 mM glycine, 10 mM Tris-Base) for 1h30 at 120 V. Membranes were blocked in a modified Tris-buffered saline + 0.05% Tween-20 (TBST) solution with 5% skim milk for 1 hr at rt, washed with TBST 3 × 5 min at rt, and were then incubated with an appropriately diluted solution of primary antibody (in 0.1% bovine serum albumin [BSA], 0.02% sodium azide, PBS pH 7.4) overnight (o/n) at 4°C. ..

    Modification:

    Article Title: The role of ATP synthase subunit e (ATP5I) in mediating the metabolic and antiproliferative effects of metformin in cancer cells
    Article Snippet: .. A quantity of 25–40 μg of protein extracts was loaded into SDS–PAGE (12–15% for resolving gels, 4% for stacking gels) and run in Tris-Glycine SDS buffer (192 mM glycine, 0.1% SDS, 25 mM Tris-Base) at 90 V. SDS–PAGE were then transferred on a nitrocellulose membrane (0.45 μm, Bio-Rad, Mississauga, ON, Canada) in Tris-Glycine buffer (96 mM glycine, 10 mM Tris-Base) for 1h30 at 120 V. Membranes were blocked in a modified Tris-buffered saline + 0.05% Tween-20 (TBST) solution with 5% skim milk for 1 hr at rt, washed with TBST 3 × 5 min at rt, and were then incubated with an appropriately diluted solution of primary antibody (in 0.1% bovine serum albumin [BSA], 0.02% sodium azide, PBS pH 7.4) overnight (o/n) at 4°C. ..

    Incubation:

    Article Title: The role of ATP synthase subunit e (ATP5I) in mediating the metabolic and antiproliferative effects of metformin in cancer cells
    Article Snippet: .. A quantity of 25–40 μg of protein extracts was loaded into SDS–PAGE (12–15% for resolving gels, 4% for stacking gels) and run in Tris-Glycine SDS buffer (192 mM glycine, 0.1% SDS, 25 mM Tris-Base) at 90 V. SDS–PAGE were then transferred on a nitrocellulose membrane (0.45 μm, Bio-Rad, Mississauga, ON, Canada) in Tris-Glycine buffer (96 mM glycine, 10 mM Tris-Base) for 1h30 at 120 V. Membranes were blocked in a modified Tris-buffered saline + 0.05% Tween-20 (TBST) solution with 5% skim milk for 1 hr at rt, washed with TBST 3 × 5 min at rt, and were then incubated with an appropriately diluted solution of primary antibody (in 0.1% bovine serum albumin [BSA], 0.02% sodium azide, PBS pH 7.4) overnight (o/n) at 4°C. ..

    Staining:

    Article Title: Use of tandem‐affinity chromatography to incorporate nucleotide loading of small GTPases during their purification
    Article Snippet: .. The next day, the nitrocellulose membrane was treated with Ponceau S stain and bands were recorded using a Bio‐Rad ChemiDoc. .. Ponceau S stain was removed from the membrane via a 0.1% NaOH solution, and then blocked for 5 min using 20 mL of Every‐Blot Blocking Buffer from Bio‐Rad.



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